Thursday, January 28, 2010

BACTERIAL GROWTH

I. Nutritional (Chemical) Requirements


A. Energy source



1. sunlight:



2. oxidation:



B. Electron Transport



1. reduction of inorganic compounds:



2. reduction of organic compounds:


C. Carbon Source

1. carbon dioxide:

2. organic materials:



II. Physical (Environmental) Requirements



A. Oxygen:



1. aerobic:



2. microaerophilic:



3. aerotolerant:



4. facultatively anaerobic:



5. obligately anaerobic: Growth in Various O2 Concentrations


















B. Temperature


1. Psychrophiles:

2. Psychrotrophs:


3. Mesophiles:


4. Thermophiles:


5. Hyperthermophiles:


C. pH


1. Acidophiles:


2. Neutrophiles:


3. Alkalophiles:


D. Pressure


1. Osmotic Pressure


a. facultative halophile:


b. extreme halophile:


2. Barametric Pressure


a. barotolerant:


b. barophile:



III. Natural vs. Laboratory Growth



IV. Processes and Measurement


A. Binary fission (log base 2)


N(final) = N(oiginal) x 2n


if n = # generations between original and final


then, log Nf = log No + n log 2,


B. Generation Time: g = t/n


where t = time between starting and final numbers.


Example









C. Measurement


1. dry weight:

2. direct count:


3. metabolic activity:


4. plate count:


5. turbidity:


Example








V. Eukaryotic Growth and Reproduction


A. Asexual-


B. Sexual-


VI. Viral Growth and Reproduction

A. Attachment:

B. Penetration:


C. Uncoating:


D. Biosynthesis:


E. Maturation:

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